Translational Diagnostics Core

The Translational Diagnostics Core provides a range of assays for human and animal hormones, peptides, and metabolites related to metabolic research. The Core is fully integrated into the high-volume Washington University Core Laboratory for Clinical Studies (CLCS).

Training of personnel and laboratory activity meets the exacting College of American Pathologists standards. Common, standardized assays of relevance to diabetes and its complications are available with outstanding quality control. The Core also assists investigators with specialized analytical testing that may be more specific to their particular diabetes-related research and contributes to method development for diabetes-related research.


Leadership

Jennifer Powers, PhD

Jennifer Powers, PhD, Director
Associate Professor of Medicine
Division of Endocrinology, Metabolism, and Lipid Research
660 S. Euclid Ave., MSC 8046-21-06
St. Louis, MO 63110
Phone 314-273-1378
Email powers.jennifer.l@wustl.edu


Other Contacts

Dave Gibson, BS
Director of Operations
Division of Endocrinology, Metabolism, and Lipid Research
660 S. Euclid Ave., MSC 8046-21-06
St. Louis, MO 63110
Phone 314- 362-7869
Email dave@dom.wustl.edu


Services

Core Diabetes Hormones and Metabolites

Human C-peptide and insulin: The principal platform for human insulin and C-peptide is the automated Roche Cobas e601. This is a chemiluminescent-based analytical system that is reliable and relatively inexpensive. Human insulin is also measured by radioimmunoassay and ELISA for investigators with special requirements.

Human pancreatic polypeptide and glucagon are quantified by radioimmunoassays.

Mouse/rat insulin are usually determined by ELISA using the Singulex Erenna (digital single molecule counting) Platform (minimum volume 5 µl). Legacy radioimmunoassay and ELISA assays for insulin and C-peptide are also performed for rat and mouse samples.

Glucose and glycated hemoglobin [HbA1c] are quantified using the automated Roche platform with colorimetric- or chemiluminescent-based enzyme and antibody-based chemistry procedures.

Adipokines

Leptin, adiponectin, IL-6, MCP-1, and TNFα assays most typically involve either RIA or ELISA methodology for quantitation. Kits are available for human as well as mouse analytes.

Lipids

Lipids and apoproteins are analyzed on the Roche Diagnostics c501 automated chemistry analyzer. Apo A1, apo B, and Lp(a) are immunoturbidometric assays. Cholesterol and triglycerides are enzymatic assays.

Direct LDL and HDL assays supplement traditional dextran sulfate precipitation methods. Fractionation of HDL into HDL2 and HDL3 is available.

FFA are quantified using an automated colorimetric assay on the Roche Diagnostics 501 analyzer.

Blood chemistry

Basic metabolic panel (BMP) and complete metabolic panel (CMP), lactate and β-hydroxybutyrate are run on a Roche Diagnostics c501 chemistry analyzer.

CBCs are run on the Beckman DxH600 hematology analyzer

Urine Chemistry

BUN, creatinine, and microalbumin performed on the Roche Diagnostics c501 chemistry analyzer.

Custom Service

Upon request, the Core will set up and run any specialty assay commercially available as a kit compatible with its instruments. These instruments include a Bio-Tek ELx 800 ELISA plate reader, a Bio-Tek Powerwave 200 plate reader, and an Aushon BioSystems Cirascan which allows a multiplex formatting with low sample volumes. The investigator pays for the kit and a small per-sample chargeback.

Send-Out Pricing

The Core contracts for provision of outside testing for a number of special assays. Contracts with Quest Diagnostics and relationships with Mayo Medical Laboratories and Barnes-Jewish Hospital Clinical Laboratories enable the Core to provide research testing at reduced rates.


Sample Submission

DRC members wishing to use the Translational Diagnostics Core fill out and email a Request for Services Form (downloadable form) to lrowe@dom.wustl.edu. The Core will provide instructions for sample submission.


Chargebacks

All tests for non-commercial research at Washington University are done at cost. Support from the Diabetes Research Center enables lower chargebacks to DRC investigators for highly utilized testing relevant to diabetes and related metabolic disorders, since DRC support is contributing to the actual cost of analyses.

Translational Diagnostics Core Chargebacks Reflecting DRC Support

TestCLSC chargebackDRC chargeback*DRC member savings/test
Insulin, cobas (human)
$13.38
$8.30
$5.08
Insulin, Immulite (human)
$18.06
$8.30
$9.76
C-peptide, cobas
$14.73
$8.36
$6.37
C-peptide, Immulite
$17.56
$8.36
$9.20
Cortisol
$14.90
$8.56
$6.34
Glucagon (RIA)
$19.22
$11.05
$8.17
Glucose
$11.29
$4.00
$7.29
Hemoglobin A1c
$15.90
$9.13
$6.77
Free fatty acids
$13.20
$7.62
$5.58
Rat/Mouse Insulin, Singulex
$21.66
$11.07
$10.59
Adipokines (Bring Your Own Kit)
RIA: leptin, adiponectin Automated: IL6, TNFa
ELISA: leptin, adiponectin, MCP1, TNFa
$15.32
$8.80
$6.32

* Chargeback rate available to DRC members. Non-members requesting testing are encouraged to apply to DRC if scientifically


In addition, because of Washington University institutional support of CLCS, the CLCS program of industry sponsored research studies, and the success of CLCS in negotiating discounted pricing with Quest Diagnostics and Mayo Medical laboratories, the Translational Diagnostics Core, through CLCS, also offers pricing for other testing that is substantially lower than can be obtained through the Barnes Jewish Hospital Laboratories. These chargebacks are available to all Washington University faculty.

Other Translational Diagnostics Core Testing*

TestBJH charge*CLCS chargeback**WUSM investigator savings/test
Alanine aminotransferase(ALT)
$16.41
$9.32
$7.09
Apolipoprotein A1 or B
$29.79
$22.94
$6.85
Basic metabolic panel
$26.12
$13.24
$12.88
Bilirubin, direct
$15.49
$8.81
$6.68
C-Peptide, RIA
NA
$12.86
NA
C-Reactive Protein (high sensitivity)
$22.97
$13.86
$9.11
CBC
$21.26
$12.08
$9.18
Comprehensive metabolic panel
$28.88
$15.57
$13.31
Creatinine, urine
$14.96
$10.96
$4.00
Estradiol RIA
$47.38
$13.28
$3.41
Free T4
$32.94
$14.15
$18.79
Growth Hormone, Immulite
$44.76
$10.13
$34.63
HDL 2/3 subfractions
NA
$30.32
NA
IGF-1
$43.58
$15.57
$28.01
Insulin, RIA
NA
$13.52
NA
LDL Direct
$21.53
$14.44
$7.09
Lipid Panel
$22.63
$14.27
$8.36
Microalbumin, urine
$20.74
$11.78
$8.96
Pancreatic Polypeptide
$85.74
$20.14
$65.60
Phosphorus
$17.46
$10.34
$7.12
Renal function panel
$31.11
$14.17
$16.94
TSH
$25.99
$13.59
$12.40
Uric acid
$11.12
$8.60
$2.52
Vitamin D, 25-Hydroxy
$47.07
$26.08
$20.99
Lactate
$16.81
$11.01
$5.80
Beta-hydroxybutyrate
$11.90
$8.82
$3.08
Ghrelin, RIA
NA
$19.52
NA
Salivary cortisol, ELISA
$32.07
$15.55
$16.52
TSH
$25.99
$13.59
$12.40

* Charges for testing through the Barnes-Jewish Hospital (BJH) laboratories at WUSM

** CLCS charges to all WUSM investigators are compared to charges of running labs through the BJH laboratories

NA, not available through BJH


Helpful Links

Core Laboratory for Clinical Studies